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Wild Blueberry Antioxidant Research Study

Published March 10th, 2009


Wild Blueberry antioxidant research results from a study showed Wild Blueberries outperformed 2 dozen other commonly consumed fruits like apples, bananas, red grapes and strawberries in an expanded test using the Cellular Antioxidant Activity (CAA) assay.

Research published in the the Journal of Agricultural and Food Chemistry (Journal Agricultural and Food Chemistry 56 (18): 8418-8426, 2008) shows that Wild Blueberries have the highest cellular antioxidant activity of the 25 fruits tested, as well as the highest total phenolic content and oxygen radical absorbance capacity (ORAC). Lead scientist Rui Hai Liu, Ph.D. used the cellular antioxidant activity (CAA) assay — a new assay developed by the Cornell University Department of Food Science to determine antioxidant activity of antioxidants, foods, and dietary supplements.

According to Dr. Liu, the CAA assay was developed to investigate how antioxidant compounds found in foods react inside cells, using human liver cancer cells as the testing model. The CAA assay takes antioxidant measurement to a new level moving beyond test tube assays to bioactivity inside cells. The CAA assay is a more biologically relevant method than the chemistry antioxidant activity assays as it accounts for uptake, metabolism, distribution and activity of antioxidant compounds within cells versus solely looking at antioxidant value. The CAA assay is the first movement in the antioxidant field away from test tube chemistry assays and toward cell-based antioxidant activity assay.

The study shows the cellular antioxidant activity of 25 common fruits, an expansion from earlier CAA testing done in 2007. (Journal of Agricultural and Food Chemistry, 2007; 55 (22), 8896-8907). “In expanding the test to include more fruits, we found that Wild Blueberries had the highest antioxidant activity, with other berries and pomegranates also showing strong performance. While further testing is needed to confirm how dietary antioxidants are absorbed by and go to work in the human body to prevent cancer and other chronic diseases, we’re encouraged by the response in this initial screening measure,” said Dr. Liu.

Wild Blueberry Association Nutrition Advisor Susan Davis, M.S., R.D. advises choosing brightly colored fruits and vegetables with an emphasis on berries. According to Davis, berries are naturally rich in anthocyanins, protective plant compounds responsible for the deep blue color of fruits like Wild Blueberries. Anthocyanins have potential antioxidant and anti-inflammatory effects, and are being studied for their possible preventative role in fighting cancer, heart disease, Alzheimer’s and other effects of aging.

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